Fig. 11. YEp-HAL3- and ppz1,2-mediated α-factor recovery depends on the presence of TRK1,2. The strains (A) W303-1A; W303-1A ppz1::URA3 ppz2::TRP1; W303-1A trk1::LEU2 trk2::HIS3; W303-1A trk1::LEU2 trk2::HIS3 ppz1::URA3 ppz2::TRP1 and (B) W303-1A [YPGE15]; W303-1A [YPGE15-HAL3]; W303-1A trk1::LEU2 trk2::HIS3 [YPGE15]; W303-1A trk1::LEU2 trk2::HIS3 [YPGE15-HAL3] were grown to an OD of 0.4 in complete media and the assay was performed as described in Materials and methods. Images of the plates were taken after 48 h and similar results were observed in four separate experiments.