Fig. 2. YEp-HAL3 and ppz1,2 strains are tolerant to toxic cations. W303-1A [pYPGE15], W303-1A [pYPGE15-HAL3], W303-1A and W303-1A ppz1::URA3 ppz2::TRP1 strains were grown to saturation in selective media, serially diluted in sterile water and spotted on to YPD plates containing the indicated amount of cations. Images were taken after 2–3 days of incubation at 28°C. Identical results were obtained from at least three independent colonies in three separate experiments, using various concentrations of each cation and in two different genetic backgrounds (DBY746 and W303-1A).