Fig. 9. ppz1,2-mediated changes in leucine uptake depends on the presence of TRK1,2. Determination of [¹⁴C]leucine uptake was performed as described (Pascual-Ahuir et al., 2001). Data are the average of three separate experiments and error bars represent the standard deviation for each point. W303-1A (filled circles); W303-1A ppz1::URA3, ppz2::TRP1 (open circles); W303-1A trk1::LEU2 trk2::HIS3 (filled squares); W303-1A trk1::LEU2 trk2::HIS3 ppz1::URA3 ppz2::TRP1 (open squares).