Fig. 3. Flag-Ccr4p co-purifies with deadenylase activity. Analysis of deadenylation activity in Flag-Ccr4p purified fractions from ccr4Δ and ccr4Δ/pop2Δ strains on a capped, polyadenylated substrate. Numbers above the lanes indicate time points taken after addition of substrate to the reaction. The arrow represents the fully deadenylated form of the substrate based on migration of substrate after cleavage with RNase H and oligo(dT) (data not show). The asterisk indicates the position of the radiolabeled phosphates.