Fig. 4. Lymphocyte development in NBS1^+/+ and NBS1^m/m mice. (A) Flow cytometric analysis of cells derived from thymus and spleen of NBS1^+/+ and NBS1^m/m mice for CD4 and CD8 surface markers. The percentage of total cells in the lymphoid gate is indicated. (B) Statistical analysis of the ratios of thymocytes, bone marrow pre-B and spleen mature B cells in NBS1^m/m and NBS1^+/m mice versus those in NBS1^+/+ mice. Data were obtained from four sets of mice. The mean value is presented with error bars. Flow cytometric analysis of cells derived from spleen and bone marrow for B220/IgM (C) and B220/CD43 (D) surface markers. The percentage of total cells residing in the lymphoid gate is indicated.