Fig. 4. Sequential conversion of the translation product into the intermediate and the processed form in a pH- and pal-dependent manner. (A–C) Wild-type or palA1 cells grown under acidic conditions (H⁺) were shifted to alkaline conditions (OH^–). Where indicated (+ CHX), cycloheximide was added 30 min before the pH shift and maintained in the alkaline media. Protein extracts taken at the indicated time points after the pH shift were analysed by western blot with α-PacC(5–265) antiserum. FL, translation product; IT, intermediate; P, processed form.