Fig. 6. Two-hybrid interaction between AN and ZWI. Top: yeast strain Y190 (–L/–W/–H) was transformed with full-length KCBP (Fl-KCBP) or Nt-KCBP in pAS1CYH2 and then transformed with AN in pACT2. Colonies were streaked on –L/–W/–H media or –L/–W media and assayed for β-galactosidase activity (A). Bottom: the yeast strain Y190 was also double-transformed with AN in pAS1CYH2 and KIPK, the non-catalytic subclone of KIPK (Sub1), the catalytic domain of KIPK (Sub 2), a KCBP-interacting putative calcium-binding protein (M6) or the motor domain of KCBP (MD) in pACT2 plasmids. The transformants were streaked on –L/–W/–H or –L/–W media. A β-galactosidase assay was performed for a replicate of each transformant (A).