Fig. 2. CKIα-RNAi stabilizes Arm protein but does not affect the rate of Arm protein synthesis. (A) Fluorogram showing incorporation of radioactivity into total cellular proteins or immunoprecipitated Arm. (B) Pulse–chase analysis of Arm protein in S2R+ cells treated with LacZ- or CKIα-dsRNA. Fluorograms of anti-Arm immunoprecipitates sampled at the indicated chase time are shown. (C) Kinetics of Arm turnover in S2R+ cells incubated with LacZ- (open squares) or CKIα-dsRNA (filled squares). Pulse–chase data for labeled Arm were quantified by a BAS-2000, with time = 0 set as 100%.