Fig. 5. Epistatic analysis showing dsh and zw3 have little effect on CKIα-RNAi-mediated Arm elevation. To cultures of S2R+ cells, 7.5 µg of LacZ-, Dsh-, ZW3- or CKIα-dsRNA were added in combinations, so that the total amount of dsRNA per culture was equal to 15 µg. After a 3 day incubation, cellular extracts were subjected to western blot analysis.