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Fig. 3. The ability of GAGA to promote long-range enhancer function is activator independent. Cells were co-transfected with either the complete reporter pGL3-prom(GAGA)Enh(GAGA/Gal4) (lanes 1–6) or the reporter lacking the proximal GAGA sites [pGL3-prom-Enh(GAGA/Gal4)] (lanes 7–12) in the presence of various combinations of expression vectors for GAGA, ΔPOZ, Gal4-ATF2 or Gal4-p53, as indicated.
