Fig. 1. (A) Amino acid sequence alignment of the TM domains of the apicomplexan microneme proteins TgMIC2, TgMIC6, TgMIC12, PbTRAP, PfTRAP, EtMIC1, Sm70, TgMIC8, TgAMA1, PfCTRP and EtMIC4. The strictly conserved amino acids are in black boxes, and the conserved residues chosen for the mutagenesis are boxed in grey. (B) Schematic representation of the constructs used in this study: MIC2myc1/2, MIC2myc2mut, MIC6Ty, MIC6Tymut and the chimeric fusions SAG1TyTMCD^MIC2, SAG1TyTMCD^MIC2mut, SAG1TyTMCD^MIC6, SAG1TyTMCD^MIC12, SAG1TyTMCD^MIC12mut, SAG1TyTMCD^MIC12* and SAG1TyTMCD^MIC12*mut. c-myc or Ty-1 were used as epitope tags and are boxed. Tg, T.gondii; Pb, P.berghei; Pf, P.falciparum; Et, E.tenella; Sm, S.muris; AMA1, apical membrane antigen 1; CTRP, circumsporozoite TRAP-related protein.