Fig. 6. SAG1TyTMCD^MIC12 is targeted to the micronemes while SAG1TyTMCD^MIC12* is re-routed to the dense granules and into the parasitophorous vacuole. Double immunofluorescence analysis by confocal microscopy was carried out with anti-MIC6 and anti-GRA3 as microneme and dense granule/parasitophorous vacuole markers, respectively. (A) SAG1TyTMCD^MIC12 was detected with anti-Ty-1 (in red) and co-localized with MIC6 (in green) (B) SAG1TyTMCD^MIC12*, carrying an out-of-frame mutation changing the sequence at the extreme C-terminus, was detected with anti-Ty-1 (in red) and co-localized with GRA3 (in green).