Skip to main content
. 2025 Oct 24;55:703–753. doi: 10.1016/j.bioactmat.2025.09.028

Table 4.

Comparative overview of crosslinking strategies in CS-SIHs.

Method Mechanism Advantages Limitations Applications Ref.
Chemical Covalent bond via bifunctional crosslinkers Strong mechanical strength, tunable degradation Residual toxicity (e.g., glutaraldehyde) Bone/cartilage TE [218]
Physical Hydrogen bonding, hydrophobic, or thermal interactions Mild, injectable, no chemical reagents Weaker mechanical properties, fast degradation Ocular, neural, or injectable systems [219]
Ionic Electrostatic interaction with counterions Rapid gelation, simple reagents pH-sensitive, less durable Nasal/ocular delivery, oral systems [220]
Enzymatic Enzyme-mediated formation of covalent bonds Biocompatible, specific, green Expensive, slower kinetics In situ gels, wound healing [221]
Radiation Free radical formation by UV/gamma rays No chemical residues, sterilization possible Requires specialized equipment Injectable systems with sterilization demand [222]