Fig. 7. Rescue of Dab2-deficient mice with Meox2–Cre. (A) Morphology of E11.5 embryos with either one functional copy of Dab2 (Dab2^fl/–) or conditionally null for Dab2 (Dab2^fl/–; Meox2^cre/+). (B) PCR analysis of P8 pups (tag number 2148–2153) born from a Dab2^fl/fl × Dab2^+/–; Meox2^cre/+ mating. The upper panel shows the Dab2 genotype, while the lower panel shows the Meox2–Cre genotype. The bottom labels indicate the genotype before and after Cre expression. (C) Immunoblot analysis of protein lysates made from the tails of mice with the genotypes indicated in (B). Forty micrograms of total protein were resolved by 10% SDS–PAGE and immunoblotted with mouse anti-Dab2 antibodies, which recognize the N-terminus common to all known Dab2 isoforms. To verify equal loading, the blot was stripped and reprobed with mouse anti-β-tubulin antibodies.