Fig. 1. Inactivation of TbMP81 expression. (A) Northern blot analysis of TbMP81 RNA from PF and BF in which RNAi was not induced (–) or was induced by growth with tet (+). RNA from the 449 parental strain cells (P) was used as a positive control, ribosomal RNA (rRNA) was used as a loading control. (B) Western analysis showing the inactivation of TbMP81 expression of upon induction of RNAi (+) in PF and BF. Cells of the parental strain and non-induced cells were used as positive controls. The cytoplasmic protein TbCSM1 (Guerra-Giraldez et al., 2002) was used as a loading control.