Fig. 4. IFN-γ-inducible BRG1 recruitment to the CIITA promoter. HeLa-Ini-11 cells were left untreated or exposed to IFN-γ for 24 h, formaldehyde cross-linked and the chromatin sonicated and used in ChIPs. Primers for CIITA promoter IV or the irrelevant PITX1 intron were used to amplify no template (lane 1), control human genomic DNA (lane 2), input DNA (lane 3) or DNA isolated following ChIP with no antibody (lane 4), anti-BRG1 (lane 5) or anti-GAL4 (lane 6). The CIITA primers amplify from –352 to +160 relative to the transcription start site.