Fig. 6. Suppression of temperature sensitivity of mgs1-18 rad18. (A) srs2 mutation suppressed the temperature sensitivity for growth of the mgs1-18 rad18Δ mutant. mgs1-18 rad18Δ srs2 cells were streaked onto YPAD and incubated at 26 (left) or 37°C (right) for 3 days. (B) srs2Δ mutation suppresses the growth defect of the mgs1Δ rad18Δ mutant. Spore colonies resulting from diploid strain TO202 were streaked onto YPAD and incubated at 26°C: (1) wild-type; (2) mgs1Δ; (3) srs2Δ; (4) rad18Δ; (5) mgs1Δ srs2Δ; (6) srs2Δ rad18Δ; (7) mgs1Δ rad18Δ; (8) mgs1Δ rad18Δ srs2Δ. (C) Increased expression of Rad52p suppresses the growth defect of mgs1-18 rad18Δ. mgs1-18 rad18Δ cells were transformed with pRS426(1), pSC52 (2) and pSC51 (3). mgs1-18 rad18Δ rad51Δ cells were transformed with pRS426 (4) and pSC52 (5). All transformants were streaked onto SC –Ura and incubated at 26 (left) or 37°C (right) for 3 days.