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. 2002 May 1;21(9):2122–2131. doi: 10.1093/emboj/21.9.2122

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Fig. 6. Size exclusion chromatography of purified HEβ. The elution profile in a Bio-Gel A column of HEβ and proteins of known Mr as standards [thyroglobulin (thy; 670 000 Da), bovine γ-globulin (ggb; 158 000 Da), chicken ovalbumin (ova; 44 000 Da), equine myoglobin (myo; 17 000 Da) and vitamin B-12 (b-12; 1350 Da)] is shown. Elution of the Mr standards was monitored by UV absorption at 280 nm (open circles), whereas HEβ was detected by western blotting with anti-E-tag mAb–POD.