Table I. Summary of interactions between proteins expressed in the yeast two-hybrid system.

Plasmid combinationsa	Appearance of colonies	Relative β-galactosidase-specific activityb
pACT2 alone	White	0.4
pACT2/1a alone	White	0.1
pAS2-1 alone	White	0.1
pAS2-1/2a alone	White	0.3
pACT2 and pAS2-1	White	0.3
pACT2/1a and pAS2-1	White	0.3
pACT2 and pAS2-1/2a	White	0.3
pACT2/1a and pAS2-1/2a	Blue	55
pBD/2a(1–126) alone	White	0.04
pACT2 and pBD/2a(1–126)	White	0.2
pACT2/1a and pBD/2a(1–126)	Blue	7.3
pACT2/p53 and pAS2-1/pSV40	Blue	3.2
pACT2/pLaminC and pAS2-1/pSV40	White	0.01

^aPlasmids are described in text and/or Materials and methods.

^bSpecific activity is calculated in micromoles of O-nitrophenyl-galactosidase hydrolysed per minute per milligram of protein.