Fig. 1. Release of deacylated tRNA from the model post-termination complex by RRF and EF-G. Release of deacylated tRNA from the model post-termination complex (1.0 A₂₆₀ units) was examined as described in Materials and methods. (A) Release of tRNA by various concentrations of RRF in the presence (open circles) or absence (closed circles) of EF-G (0.5 µM). (B) Release of tRNA by various concentrations of EF-G alone. The percentage tRNA release relative to the total number of deacylated tRNAs bound to the model post-termination complex (1838 c.p.m., measured by the aminoacylation with [¹⁴C]amino acids mixture) was plotted against the amount of added RRF (A) or EF-G (B).