Fig. 5. Model for disassembly of the post-termination complex by EF-G and RRF. (A) RRF binds to the A-site of the post-termination complex. EF-G binds to the factor-binding site. (B) RRF is translocated (or makes a certain movement, see Discussion) by EF-G, resulting in the release of tRNA from the complex. Two tRNAs, one each from the P- and E-sites, are presumed to be released in this step. This step is sensitive to thiostrepton, viomycin and aminoglycosides. (C) The next step is the release of mRNA, RRF and EF-G. EF-G release is required for the release of mRNA from the ribosome, hence fusidic acid and GMPPCP inhibit this step.