Table II. Comparison of concentrations of inhibitors required for 50% inhibition of the release of tRNA and mRNA from the model post-termination complex by RRF and EF-G.
| Inhibitors | Concentration required for 50% inhibition |
|
|---|---|---|
| tRNA release | mRNA release | |
| Thiostrepton | 92.3 µM | 12.5 µM |
| Viomycin | 58.3 µM | 25.0 µM |
| Fusidic acid | No inhibition | 10.0 µMa |
| Paromomycin | 11.4 µM | 1.2 µM |
| Gentamicin | 2.1 µM | 0.5 µM |
| Streptomycin | 68.5 µM | 25.0 µMa |
| Tobramycin | Not tested | 3.7 µM |
| Neomycin | Not tested | 1.7 µM |
| G418 | Not tested | 63.0 µM |
| Kanamycin | Not tested | 20.4 µM |
aThese values were estimated from Hirashima and Kaji (1973).