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Fig. 4. WSTF forms a complex with ISWI in Xenopus egg extracts. (A) Western blot of co-purification of Xenopus WSTF (anti-human WSTF antibodies) and Xenopus ISWI (anti-Xenopus ISWI antibodies) over MonoS chromatography during ISWI-B purification. This is the penultimate step in the purification scheme. (B) SDS–PAGE of the final purified complex showing the two polypeptides. Immunoblot shows the antibody reactivity with the respective antisera.
