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. 2025 Nov 10;26(6):bbaf586. doi: 10.1093/bib/bbaf586

Figure 2.

Series of UMAP plots comparing PBMC datasets from different technologies projected onto a reference blood atlas using various methods, with boxplots and a Sankey diagram illustrating integration quality and label consistency.

Mapping PBMC query data from different technologies to DISCO blood atlas. (a) Uniform manifold approximation and projection (UMAP) plots showing the blood atlas in DISCO database. Each dot represents a single cell, colored by cell type. (b, c). Projection of PBMC datasets from three 10x protocols onto DISCO blood atlas in (a) left, UMAP plots without batch correction; middle, UMAP plots by harmony; right, query cells projected by ProjectSVR. Dots were colored by protocols (b) and published cell types identified by Korsunsky et al. (d) Boxplot showing the distribution of LISI (local inverse Simpson’s index) across different integration or reference mapping methods. (e) Transfer cell labels from blood atlas (reference) to query cells using a 10-NN classifier on the ProjectSVR embedding. Query cells were colored by cell type defined in (a), and grey dots represent reference cells. (f) Sankey diagram shows the consistency between predicted cell labels in (e) and published cell labels in (c).