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RT-PCR analysis of some distinctly over expressed genes in EB. RT-PCR analysis of some distinctly overexpressed genes in EB as identified by microarray analysis. Marked overexpression in EB is clearly documented in the figure. Total RNA derived from both the ES and EB cell lines (BG02 and WiCell lines) were subjected to RT-PCR analysis as described in Fig. 2 legend. G3PDH mRNA amplified from these samples served as an internal control.
