Fig. 5. In situ localization of human U87, U88 and U89 box C/D–H/ACA RNAs. Human U87, U88 and U89 RNAs were transiently over expressed in HeLa cells using the pCMV-globin expression construct. The intracellular distribution of the overproduced U87, U88 and U89 RNAs as well as the endogenous HeLa U88 RNA (U88endo) was investigated by fluorescent in situ hybridization. Cajal bodies were visualized by staining with anti-p80 coilin antibody. For other details, see the legend to Figure 4.