Fig. 3. Binding of preprotein to Tic110 and mutant proteins reconstituted into liposomes. (A) After binding, ³⁵S-labelled pSSU was re-isolated together with inner envelope vesicles (5 µg protein), Tic110-proteoliposomes and ΔN-proteoliposomes (50 ng protein), but was not recovered together with ΔC-proteoliposomes. After binding and re-isolation, half of each sample was treated with thermolysin (Thl). The translation product (20%) used for binding is shown in the left lane (–). (B) As a control, the same experiment was performed with liposomes that did not contain protein, but were treated like proteoliposomes. The translation product (20%) used for binding is shown in the left lane (–). Binding was examined by 25% High-Tris–Urea PAGE. X-ray films are shown.