Fig. 2. los2 blocks cold induction of COR/KIN/RD/LTI but not CBF genes. (A) Endogenous RD29A expression in wild-type and los2 plants under different stress treatments. (B) The expression of other stress-responsive genes in wild-type and los2 plants. Wild-type and los2 plants were grown in the same agar plates for 3 weeks under continuous light at 22 ± 2°C. The seedlings were then treated with cold (0 ± 0.1°C for 24 h), ABA (100 µM for 3 h), NaCl (300 mM for 3 h) or PEG (30% for 5 h). Genes for β-tubulin or actin were used as loading controls. CT, control; C, cold; A, ABA; N, NaCl; P, PEG.