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. 2002 Jun 3;21(11):2692–2702. doi: 10.1093/emboj/21.11.2692

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Fig. 3. Chilling and freezing sensitivity in los2 plants. (A) Morphology of wild-type and los2 plants with or without cold treatment and grafting experiment. (a) Wild-type and los2 plants are normal when grown at 22°C. (b and d) los2 but not wild-type plants wilt when incubated under light (20 µmol/m2/s) at 4°C for 7 days. (c and e) Neither los2 nor wild-type plants wilt when incubated in the dark at 4°C for 7 days. (f–j) Grafting experiment showing that wilting is not caused by the los2 root. (f) A successfully grafted plant. The circle highlights the graft junction. (g–j) Chilling sensitivity of grafted plants at 4°C. Grafted plants in soil were incubated in the cold room for 10 days under continuous light (20 µmol/m2/s) before pictures were taken. (g) Seedling with a wild-type shoot and los2 root. (h) Seedling with a los2 shoot and root. (i) Seedling with a los2 shoot and wild-type root. (j) Seedling with a wild-type shoot and root. (B) Morphology of wild-type and los2 plants after freezing stress, with or without cold acclimation treatment. (C) Quantification of the freezing survival rate for wild-type and los2 plants with cold acclimation treatment.