Fig. 9. STZ/ZAT10 can repress the expression of RD29A in Arabidopsis leaves. (A) Schematic representation of the reporter and effector plasmids used in transient expression assays. A portion of the RD29A promoter (black oval) was fused to firefly luciferase. Nos denotes the terminator signal of the gene for nopaline synthase. Ω indicates the translational enhancer of tobacco mosaic virus. (B) Relative luciferase activities after transfection with RD29A-LUC and 2× 35S-STZ/ZAT10. To normalize values obtained after each transfection, pPTRL DNA that contained the CaMV 35S promoter and a gene for luciferase from Renilla was used as internal control. Luciferase activity was expressed in arbitrary units relative to the activity of Renilla luciferase (as described in Ohta et al., 2001). The values are averages of three bombardments, and error bars indicate standard deviations.