Fig. 3. Specific E/EC1–2 bead self-aggregation. (A) Representative field of E/EC1–2-coated beads in the presence of CaCl₂ or EDTA (bar = 5 µm). (B) Beads were allowed to aggregate in the presence of Ca²⁺ or EDTA. The aggregation index was high for E/EC1–2 beads, but not for E/W2A or control beads (casein or BSA). E/EC1–2 bead aggregation was Ca²⁺ dependent. Data are mean values of at least two independent experiments (∼4500 beads counted for each condition).