Reagents	Final concentration	Amount
1 M HEPES-KOH, pH 7.5	50 mM	25 mL
5 M NaCl	100 mM	10 mL
0.5 M EDTA, pH 8	1 mM	1 mL
0.5 M EGTA, pH 8	0.5 mM	0.5 mL
Milli-Q water	N/A	463.5 mL
Total	–	500 mL