Reagents	Final concentration	Amount
1 M HEPES-KOH, pH 7.5	50 mM	25 mL
5 M NaCl	140 mM	14 mL
0.5 M EDTA, pH 8	1 mM	1 mL
Glycerol	10% (v/v)	50 mL
NP-40 alternative	0.5% (v/v)	2.5 mL
Triton X-100	0.25% (v/v)	1.25 mL
Milli-Q water	N/A	∼406.25 mL
Total	–	500 mL