Reagents	Final concentration	Amount
1 M Tris-HCl, pH 7.5	25 mM	1.25 mL
0.5 M EDTA, pH 8	5 mM	0.5 mL
10% (v/v) SDS	0.5% (v/v)	2.5 mL
Milli-Q water	N/A	∼45.75 mL
Total	–	50 mL