Fig. 4. Phosphorylation of the Tyr15 in Cdc2 is extended in pat1 meu13Δ. pat1 (JZ670), pat1 meu13Δ (KN8), pat1 rec12Δ meu13Δ (MS110–2), pat1 rad17Δ meu13Δ (MS107-1), pat1 rec12Δ (MS123-5) and pat1 rad17Δ (MS101-4) cells were induced to enter meiosis as shown in Figure 1. Samples were taken after the temperature shift at the indicated time points and western blot analysis was performed to detect the amount of Cdc2 and phosphorylated Cdc2.