Fig. 1. Sequence analysis of Kin3. (A) Domain organization of Kin3 and close relatives. Kin3 contains all domains typical for members of the UNC-104/KIF1 family. This includes the N-terminal motor domain (red), an FHA domain (yellow), a PH domain (blue) and short coiled-coil regions (green). (B) Dot-plot comparison of the predicted amino acid sequence of Kin3 versus MmKIF1A. Note that both motors share sequence similarity along the entire length of the molecule. (C) Nearest-neighbor dendrogram of potential kinesin organelle transporters. Kin3 groups with members of the UNC-104/KIF1 family. The dendogram is based on a distance matrix-based neighbor-joining analysis of the kinesin motor domain. Statistical support for this tree was obtained by bootstrapping (neighbor joining and parsimony, 1000 resamplings each) or Quartet Puzzling. Solid circles indicate a bootstrap and QP support of >80%, while open circles indicate 60–80% support. Accession numbers are given. The bar reflects 0.1 changes per amino acid.