Fig. 8. Distribution of chitin and bud-site selection during the cell cycle of U.maydis. (A) Chitin was stained with WGA in strain FB2. Chitin accumulates strongly at the bud scars in all stages of the cell cycle (arrow in A1–A7). The new bud emerges at the cell pole opposite the bud scar (A2, A3), and chitin localizes to the tip and to the neck region (arrowheads in A3). In cells with larger buds, the chitin ring at the neck becomes more faint, while the tip still carries chitin (arrowhead in A4). Subsequently, chitin appears at the septa (arrowhead in A5), and two bud scars remain after cell separation (pair of arrows in A6). The next appears next to the old bud scar (arrowhead in A7), finally leading clusters of ring-like bud scars (small image in A7) at both ends of the cell. Bar: 3 µm. (B) In Δkin3 cells, many bud scars accumulate at one cell pole (arrows). Bar: 3 µm.