Table I. Strains and plasmids used in this study.
| Strain | Genotype | Reference |
|---|---|---|
| FB1 | a1b1 | Banuett and Herskowitz (1989) |
| FB2 | a2b2 | Banuett and Herskowitz (1989 |
| RWS4 | a1b1 /pYup1SG2 | Wedlich-Söldner et al. (2000) |
| FB1otefGFPtub1 | a1b1 /potefGFPTub1 | Steinberg et al. (2001) |
| RWS6 | a2b2 /pKin3GFP1 | This study |
| RWS7 | a2b2 /pKin3GFP2 | This study |
| RWS8 | a1b1 /pKin3YFP /pCFPTub1 | This study |
| RWS9 | a1b1 /pKin3YFP /pPX10-146CFP | This study |
| RWS10 | a2b2 Δkin3::hphR /pYup1SG3 | This study |
| RWS11 | a2b2 Δkin3::hphR /pRU4-Kin3 /pYup1SG3 | This study |
| RWS12 | a2b2 Δkin3::hphR /potefGFPTub1 | This study |
| RWS13 | a2b2 Δkin3::hphR /potefGFPTub1 /pYup1SG3 | This study |
| FB1Dyn2ts | a1b1 Δdyn2::[dyn2ts, natR] | Wedlich-Söldner et al. (2002) |
| RWS14 | a1b1 Δdyn2::[dyn2ts, natR] Δkin3::hphR /pYup1SG2 | This study |
| RWS15 | a1b1 Δdyn2::[dyn2ts, natR] /pYup1SG2 | This study |
| RWS16 | a2b2 Pcrg-kin3, natR /pYup1SG2 | This study |
| RWS17 | a2b2 Pcrg-kin3, natR /potefGFPTub1 | This study |
| RWS18 | a2b2 Δkin3::hphR | This study |
| RWS19 | a2b2 Δkin3::hphR /pYup1SG3 /pcrgKin3G105E | This study |
| RWS20 | a2b2 Δkin3::hphR /pYup1SG3 /pcrgKin3 | This study |
| pKin3GFP1 | Pkin3-kin3-egfp, cbxR | This study |
| pKin3GFP2 | Potef-kin3-egfp, cbxR | This study |
| pKin3YFP | Potef-kin3-yfp, cbxR | This study |
| pCFPTub1 | Potef-cfp-tub1, hygR | This study |
| pPX10-146CFP | Potef-yup1PX1-146-cfp, hygR | This study |
| PYup1SG3 | Potef-yup1-sgfp, bleR | This study |
| pRU4-Kin3 | Pkin3-kin3, cbxR | This study |
| pcrgKin3 | Pcrg-kin3, cbxr | This study |
| pcrgKin3G105E | Pcrg-kin3G105E, cbxr | This study |
| pET-Kin3U1 | PT7-Kin31–358 | This study |
| pET-Kin3U2 | PT7-Kin31–431 | This study |
| potefGFPTub1 | Potef-egfp-tub1, cbxR | Steinberg et al. (2001) |
a, b, mating type loci; Δ, deletion; P, promoter; ::, homologous replacement; -, fusion; ts, temperature-sensitive allele; hphR, hygromycin resistance; bleR, phleomycin resistance; natR, nourseothricin resistance; cbxR, carboxin resistance; /, ectopically integrated; yup1PX1-146, localization domain of the endosomal t-SNARE Yup1 (amino acids 10–146); gfp, green fluorescent protein; yfp, yellow-shifted derivative of green fluorescent protein; cfp, cyan-shifted derivative of green fluorescent protein; Kin31–358 and Kin31–431, motor domain constructs of Kin3; tub1, α tubulin; dyn2, gene encoding the C-terminal third of the dynein heavy chain.