Fig. 5. ‘Lineage switch’ from macrophage to megakaryocyte by GATA-2. (A) Morphology of the cells at day 9 of induction in the presence of M-CSF. The characteristics of macrophages were lost in the absence of tetracycline. (B and C) Expression of Mac-1 and platelet GPV in the presence or absence of tetracycline. (D) Cell numbers of control (P > 0.05 by t-test) and GATA-2 clones (*P < 0.01) in the presence or absence of tetracycline at day 9 of induction with M-CSF. (E) Western blot analysis of GATA-1, PU.1 and GATA-2 of control E14tg2a ES cells and GATA-2 clones in the presence or absence of tetracycline.