Fig. 6. Effects of GATA-1 and the activated GATA-2/ER on hematopoietic colony formation and differentiation. (A) Effects on day 7 colony formation. A significant reduction in the cell number by the enforced expression of GATA-2/ER was observed in the presence of β-estradiol (*P < 0.01 by t-test). (B) Effects of GATA-1 and the activated GATA-2/ER on erythrocyte production in the presence of EPO. GATA-1 and the activated GATA-2/ER gave rise to a significant increase and decrease of the erythroid lineage cells, respectively (*P < 0.01, #P < 0.05 by t-test). Data are shown as the mean ± SD of six samples. (C) Western blot analyses of GATA-2 and GATA-2/ER in the day 8 differentiation-induced cells. (D and E) Expression of Mac-1 and platelet GPV at day 9 of culture in the presence of M-CSF. (F) Expression of megakaryocyte-specific acetylcholine esterase by overexpression of GATA-1 and GATA-2.