Table I. Reversal of prohibitin-induced repression of colony formation by dnBrg-1 and dnBrma.
| Vector transfected | No. of colonies |
|||
|---|---|---|---|---|
| B05-1 (dnBrg-1) |
H-16 (dnBrm) |
|||
| Experiment 1 | Experiment 2 | Experiment 1 | Experiment 2 | |
| PCDNA3 + pBabe.puro + Tet | 234 | 225 | 203 | 209 |
| PCDNA3 + pBabe.puro – Tet | 238 | 232 | 219 | 217 |
| PCDNA3Prohibitin + pBabe.puro + Tet | 31 | 36 | 53 | 59 |
| pCDNA3Prohibitin + pBabe.puro – Tet | 224 | 215 | 198 | 205 |
aApproximately 10 000 B05-1 or H-16 cells were transfected with 2 µg of the indicated vectors. Colonies with ≥20 cells were counted after 14 days of selection in 1 µg of puromycin per ml.