Fig. 4. Fluorescence microscopy of exponentially growing cells from strains (A) PG35 (scpB-yfp, spo0J::spec), (B) PG33 (scpB-yfp, pspac-smc at amy locus, smc::kan), (C) JM17 (scpA-yfp, pspac-smc at amy locus, smc::kan), (D) PG41 (scpA-yfp, scpB::tet, pxyl-scpB-cfp at amy locus), (E) JM10 (pxyl-scpB-cfp at amy locus) and (F) JM18 (scpA::tet, pxyl-scpB-cfp at amy locus) in the presence of xylose. Depleted indicates that the inducer was removed during exponential growth 3–5 h prior to image acquisition. Arrows indicate anucleate cells in (A) and ScpB–CFP foci in (E), and white lines indicate septa between cells. Images ‘induced’ and ‘depleted’, and (E) and (F) are scaled. White rectangles indicate 2 µm.