Table I. List of plasmids.
Genotypea |
minE alleles |
|||
---|---|---|---|---|
minEWT | minED45A | minEV49A | minED45A/V49A | |
Plac-minC minD minE | pSY1083b | pYLS4 | pYLS40 | |
Plac-minC minD minE::gfp | pSY1083Gb | pYLS4G | pYLS40G | pYLS76G |
Plac-gfp::minD minE | pFX9c | pYLS33 | pYLS46 | pYLS92d |
Plac-yfp::minD minE::cfp | pYLS68 | pYLS69 | pYLS77 |
aThe presence of the desired mutations and the absence of min mutations elsewhere were confirmed by sequencing the entire min locus of the plasmids.
dContains yfp in place of gfp.