Fig. 2. PCR products generated with lariat-specific primer sets using as template barley DNA (D; control) or cDNAs raised from barley RNA with various treatments [C, untreated RNA; prior incubation in ligase buffer without (LU) or with (H, LH) heat denaturation, and addition of RNA ligase (LH, LU)], and were separated on agarose gels along with marker DNA (M). Major bands from lanes C and H were of sizes estimated for lariat formation at the conserved adenosine in D6 (petB, 161 bp; petD, 231 bp; rps12 trans, 161 bp; trnG, 229 bp). Additional larger bands specific to lanes LH and LU proved successful RNA circularization (see text).