Fig. 2. p38 is required for TGF-β-induced EMT. NMuMG cells were incubated with the p38 kinase inhibitor SB203580 (E–H) or MEK1/2 inhibitor U0126 (I–L) in the absence (A, B, E, F, I and J) or presence of TGF-β (C, D, G, H, K and L) for 36 h, followed by staining for the actin cytoskeleton and E-cadherin. Control cells in the absence of protein kinase inhibitor (A–D).