Fig. 3. Light-induced RNAs determined by northern blot analysis. (A–C) frq RNA levels; (D–F) vvd RNA levels. (A) and (D) are samples from wild-type cultures; (B) and (E) are from Δwc-1; and (C) and (F) are from Δwc-2, where the panels are overexposed. Loading of RNA was controlled by probing for rRNA (not shown), and these values were used for quantification. (G) Quantification of frq RNA induction as shown in (A) and (B). The harvest times are indicated, up to 6 h after lights-on, with DD28 (DD indicates constant darkness, e.g. 28 h) and DD34 representing the dark controls harvested at the beginning and the end of the light exposure.