Table I. In vivo recombination at psi and psiDC in E.coli strains carrying mutant xer genes.
Plasmid | Genotype |
|||
---|---|---|---|---|
xerC+ xerD+ | xerC+ xerDYF | xerCYF xerD+ | xerCYF xerDYF | |
p-psi.psi | +++ | +++ | – | – |
p-psiDC.psiDC | ++ | – | + | – |
Plasmids were transformed into strains carrying the chromosomal copies of xer alleles indicated. The extent of recombination observed by agarose gel electrophoresis after overnight growth is indicated as: +++, 100% of substrate converted to product; ++, 50% of substrate converted to product; +, 15% of substrate converted to product; –, no detectable recombination.