Fig. 7. Effect of the lack of ζPKC on AP-1 and NF-κB activation in B cells. Nuclear extracts from either wild type (empty bars) or ζPKC-deficient (black bars) B cells that have been stimulated or not with 20 µg/ml IgM for 90 min were analyzed by EMSA using an AP-1 probe (A). Gels of three experiments like the one shown in (A) were quantified in an InstantImager, and the mean ± SD of the CPMs of the AP-1 bands are shown in (B). The experiment shown in (C) corresponds to nuclear extracts from stimulated B cells as above but which have been pre-incubated or not with an anti-c-Fos antibody. The supershifted band corresponding to the c-Fos-containing AP-1 complex is shown as SS. These are representative experiments of another two with similar results. In a parallel experiment, nuclear extracts from either wild-type or ζPKC-deficient B cells that have been stimulated or not with 20 µg/ml IgM with or without 10 ng/ml BAFF for 90 min were analyzed by EMSA using a κB probe (D). This is a representative experiment of at least another three with similar results.