Fig. 8. Effect of the lack of ζPKC on the transcription of NF-κB-dependent genes. Wild-type (empty bars) and ζPKC-deficient (black bars) B cells were stimulated with 20 µg/ml IgM for 1.5 and 4 h, after which RNA was extracted and the levels of IκB (A), IL-6 (B), Bcl-XL (C) and 18S (A, B and C) RNAs were determined by RT–PCR with the appropriate primers. The inset panels show representative experiments of another two with similar results, and the lower panels show the quantification of that experiment.