Figure 1.

Intrapulmonary arteries. A) laser-microdissection of small intrapulmonary arteries. 1) The laser cuts along the outer side of the tunica adventitia. 2) A sterile needle is used to isolate the vessel. 3) Needle with adherent vessel is lifted and transferred afterwards to a reaction tube. Magnification × 200. B) Representative intrapulmonary arteries during the process of vascular remodelling. 1) Under normoxic conditions. 2) At day 1 of hypoxia. 3) At day 7 of hypoxia. Smooth muscle cell layer causes vascular thickening. 4) At day 21 of hypoxia. Magnification × 200.